Composite

Part:BBa_K5377830:Design

Designed by: Jiyue Hu   Group: iGEM24_ZJUT-China   (2024-09-21)


TPP self-induced production system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2829
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2829
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2829
    Illegal BglII site found at 3266
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2829
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2829
    Illegal AgeI site found at 1824
    Illegal AgeI site found at 3156
    Illegal AgeI site found at 3387
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. The thiM TPP riboswitch is from the Escherichia coli. If you want to use it in other strains, please ensure that the riboswitch RBS sequence can be recognized.
2. The titer of TPP has only been validated in Escherichia coli.

Source

thiM TPP riboswitch is from E. coli.
thiL, thiC, thiM are from E. coli.
The pHG101 plasmid (contain J23106 promoter) is synthesized by RuiBiotech Co., Ltd.

References

[1]Rentmeister, A., Mayer, G., Kuhn, N., & Famulok, M. (2007). Conformational changes in the expression domain of the Escherichia coli thiM riboswitch. Nucleic acids research, 35(11), 3713–3722. https://doi.org/10.1093/nar/gkm300
[2]Sudarsan, N., Cohen-Chalamish, S., Nakamura, S., Emilsson, G. M., & Breaker, R. R. (2005). Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine. Chemistry & biology, 12(12), 1325–1335. https://doi.org/10.1016/j.chembiol.2005.10.007
[3]Thore, S., Leibundgut, M., & Ban, N. (2006). Structure of the eukaryotic thiamine pyrophosphate riboswitch with its regulatory ligand. Science (New York, N.Y.), 312(5777), 1208–1211. https://doi.org/10.1126/science.1128451
[4] JACOB, F., & MONOD, J. (1961). Genetic regulatory mechanisms in the synthesis of proteins. Journal of molecular biology, 3, 318–356. https://doi.org/10.1016/s0022-2836(61)80072-7
[5] Müller-Hill, B. (1996). The lac Operon: A Short History of a Genetic Paradigm. Berlin, New York: De Gruyter. https://doi.org/10.1515/9783110879476